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 Metagenome
bank |
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| Construction
of the Microbial diversity and metagenome library from tidal flat
sediments, Saemankum. |
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| Although microorganisms account for 60 % of the organism on earth,
only less than 1 % of microorganisms in nature can be cultivated in
the laboratory. Therefore, this research team have done systematical
taxonomy of cultivable microorganisms from extreme environment such
as sea water, tidal flat sediments, high salt or alkali soils. In
order to search for uncultivable microorganisms and genomic resources,
we have developed new screening techniques and constructed metagenomic
library from various environment. Moreover, we have isolated and obtained
the myxobacteria, which are known to produce a useful secondary metabolites
as the actinomycetes. And we have established the phylogenetic system
of the myxobacteria. (Figures give an example of the Microbial diversity
(1), Myxobacteria (2), and screening of lipase from Metagenomic libarary(3)) |
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| Proteome
bank |
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 Proteombank is a dedicated protein-science team which is defining the function
of proteins at the tertiary structure level. Currently, a major focus
of our activity is the examination of the protein-small molecule interactions.
Also, our team is working that is collecting and sales of protein
resource from sequenced microbial genomic library. |
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| Metabolome
bank |
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Distribution of microbial metabolmes |
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supplied microorganism-derived metabolites from domestic and abroad,
we contribute to find lead molecule and redesign of metabolic pathway. |
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Expression, extraction, isolation and identification of metabolome
derived from microorganisms |
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Distribution of microbial metabolites from microbial genomes,
fraction metabolome and compound metabolome |
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Screening for bioactive compounds from microorganisms and determination
of the biosynthetic pathways |
| My researches are focused on the screening for useful bioactive
compounds (antibiotic, anticancer, etc.) from microorganisms, and
the determination of their chemical structure, mechanism of physiological
activity, and biosynthetic pathway. Based on the research data, the
biosynthetic genes and/or pathway should be engineered to produce
new bioactive analogues. |
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Construction of MS/MS spectra library using LC-MS |
| Searchable MS/MS spectra libraries, constructed using the results
of liquid chromatography-tandem mass spectrometry (LC-MS/MS) with
data-dependent acquisition on an ion trap mass spectrometer coupled
with electrospray ionization (ESI), are here presented with regard
to the identification and confirmation of a variety of metabolites
in a set of biological samples. |
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Screening of useful clones from various metagenome libraries |
| Secondary metabolites produced by microbes have been important sources
of bioactive molecules such as antibiotics and other pharmaceutical
agents over the last 60 years. However, the discovery rate of novel
biomolecules using traditional cultivation techniques has significantly
decreased during the past couple of years. Recent study in molecular
microbiology has shown that more than 99% of the microorganisms present
in many natural environments are not readily culturable with currently
available technique. Thus, these uncultured microorganisms are unexplored
reservoir of novel strains which may produce novel natural products.
Culture-independent method developed to exploit the genomes of uncultured
majority is the metagenome technology, which has provided access to
the untapped genomes of uncultured microorganism. We constructed metagenome
libraries form various environmental sample. We find clones which
produce useful secondary metabolites using by activity-based method
and sequence-based method. |
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Metabolomic study of myxobacteria |
Recently research trend for bioactive compound discovery is observing
new compound detection from specific microorganisms. Among them myxobacteria
are producers of novel bioactive secondary metabolites.
So, we work up secondary metabolite pattern analysis and novel natural
products discovery of myxobacteria 600 wild strain by taxonomy classify |
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52,
Oun-dong, Yusong-gu, Taejon, 305-333, Korea